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991.
Sadovy de Mitcheson Yvonne J. Mitcheson George R. Rasotto Maria B. 《Environmental Biology of Fishes》2022,105(6):699-716
Environmental Biology of Fishes - Mating systems in fishes are extremely diverse, ranging from monogamy to multiple polygamous forms, some of which include alternative male mating tactics within... 相似文献
992.
Heike I. Rsner Martina Caldarini Gregory Potel Daniel Malmodin Maria A. Vanoni Alessandro Aliverti Ricardo A. Broglia Birthe B. Kragelund Guido Tiana 《Proteins》2022,90(1):96-109
The denatured state of several proteins has been shown to display transient structures that are relevant for folding, stability, and aggregation. To detect them by nuclear magnetic resonance (NMR) spectroscopy, the denatured state must be stabilized by chemical agents or changes in temperature. This makes the environment different from that experienced in biologically relevant processes. Using high-resolution heteronuclear NMR spectroscopy, we have characterized several denatured states of a monomeric variant of HIV-1 protease, which is natively structured in water, induced by different concentrations of urea, guanidinium chloride, and acetic acid. We have extrapolated the chemical shifts and the relaxation parameters to the denaturant-free denatured state at native conditions, showing that they converge to the same values. Subsequently, we characterized the conformational properties of this biologically relevant denatured state under native conditions by advanced molecular dynamics simulations and validated the results by comparison to experimental data. We show that the denatured state of HIV-1 protease under native conditions displays rich patterns of transient native and non-native structures, which could be of relevance to its guidance through a complex folding process. 相似文献
993.
Tatarkova Zuzana Bencurova Maria Lehotsky Jan Racay Peter Kmetova Sivonova Monika Dobrota Dusan Kaplan Peter 《Molecular and cellular biochemistry》2022,477(5):1621-1628
Molecular and Cellular Biochemistry - Increased concentration of plasma homocysteine (Hcy) is an independent risk factor of cardiovascular disease, yet the mechanism by which hyperhomocysteinemia... 相似文献
994.
Perrone Maria Rita Romano Salvatore De Maria Giuseppe Tundo Paolo Bruno Anna Rita Tagliaferro Luigi Maffia Michele Fragola Mattia 《Aerobiologia》2022,38(3):391-412
Aerobiologia - The SARS-CoV-2 presence and the bacterial community profile in air samples collected at the Intensive Care Unit (ICU) of the Operational Unit of Infectious Diseases of Santa Caterina... 相似文献
995.
Gharbi Dorra Mobayed Hassan M. Ali Ramzy Mohammed Tuffaha Amjad Dason Blessing Reena Ibrahim Tayseer Adeli Mehdi Sattar Hisham A. Trigo Maria del Mar Al-Nesf Maryam Ali 《Aerobiologia》2022,38(3):329-342
Aerobiologia - Daily monitoring of airborne fungal spores was carried out for the first time in Al Khor city, Qatar, using a Hirst type 7-day recording volumetric spore trap, from May 2017 to May... 相似文献
996.
997.
Markus Oberpaul Stephan Brinkmann Michael Marner Sanja Mihajlovic Benedikt Leis Maria A. Patras Christoph Hartwig Andreas Vilcinskas Peter E. Hammann Till F. Schäberle Marius Spohn Jens Glaeser 《Microbial biotechnology》2022,15(2):415-430
High-throughput platforms facilitating screening campaigns of environmental samples are needed to discover new products of natural origin counteracting the spreading of antimicrobial resistances constantly threatening human and agricultural health. We applied a combination of droplet microfluidics and fluorescence-activated cell sorting (FACS)-based technologies to access and assess a microbial environmental sample. The cultivation performance of our microfluidics workflow was evaluated in respect to the utilized cultivation media by Illumina amplicon sequencing of a pool of millions of droplets, respectively. This enabled the rational selection of a growth medium supporting the isolation of microbial diversity from soil (five phyla affiliated to 57 genera) including a member of the acidobacterial subgroup 1 (genus Edaphobacter). In a second phase, the entire diversity covered by 1071 cultures was used for an arrayed bioprospecting campaign, resulting in > 6000 extracts tested against human pathogens and agricultural pests. After redundancy curation by using a combinatorial chemical and genomic fingerprinting approach, we assigned the causative agents present in the extracts. Utilizing UHPLC-QTOF-MS/MS-guided fractionation and microplate-based screening assays in combination with molecular networking the production of bioactive ionophorous macrotetrolides, phospholipids, the cyclic lipopetides massetolides E, F, H and serratamolide A and many derivatives thereof was shown. 相似文献
998.
Nuria Pujol-Carrion Alma Gonzalez-Alfonso Sergi Puig Maria Angeles de la Torre-Ruiz 《Microbial biotechnology》2022,15(5):1525-1541
Ferritin proteins have an enormous capacity to store iron in cells. In search for the best conditions to accumulate and store bioavailable iron, we made use of a double mutant null for the monothiol glutaredoxins GRX3 and GRX4. The strain grx3grx4 accumulates high iron concentrations in the cytoplasm, making the metal easily available for ferritin chelation. Here, we perform a comparative study between human (L and H) and soya bean ferritins (H1 and H2) function in the eukaryotic system Saccharomyces cerevisiae. We demonstrate that the four human and soya bean ferritin chains are successfully expressed in our model system. Upon coexpression of either both human or soya bean ferritin chains, respiratory conditions along with iron supplementation led us to obtain the maximum yields of iron stored in yeast described to date. Human and soya bean ferritin chains are functional and present equivalent properties as promoters of cell survival in iron overload conditions. The best system revealed that the four human and soya bean ferritins possess a novel function as anti-ageing proteins in conditions of iron excess. In this respect, both ferritin chains with oxidoreductase capacity (human-H and soya bean-H2) bear the highest capacity to extend life suggesting the possibility of an evolutionary conservation. 相似文献
999.
Thomas Clavel Hans-Peter Horz Nicola Segata Maria Vehreschild 《Microbial biotechnology》2022,15(1):164-175
Gut microbiome research has bloomed over the past 15 years. We have learnt a lot about the complex microbial communities that colonize our intestine. Promising avenues of research and microbiome-based applications are being implemented, with the goal of sustaining host health and applying personalized disease management strategies. Despite this exciting outlook, many fundamental questions about enteric microbial ecosystems remain to be answered. Organizational measures will also need to be taken to optimize the outcome of discoveries happening at an extremely rapid pace. This article highlights our own view of the field and perspectives for the next 15 years. 相似文献
1000.
Therese S. Høiem Maria K. Andersen Marta Martin-Lorenzo Rémi Longuespée Britt S.R. Claes Anna Nordborg Frédéric Dewez Benjamin Balluff Marco Giampà Animesh Sharma Lars Hagen Ron M.A. Heeren Tone F. Bathen Guro F. Giskeødegård Sebastian Krossa May-Britt Tessem 《Proteomics》2022,22(10):2100223
MALDI MS imaging (MSI) is a powerful analytical tool for spatial peptide detection in heterogeneous tissues. Proper sample preparation is crucial to achieve high quality, reproducible measurements. Here we developed an optimized protocol for spatially resolved proteolytic peptide detection with MALDI time-of-flight MSI of fresh frozen prostate tissue sections. The parameters tested included four different tissue washes, four methods of protein denaturation, four methods of trypsin digestion (different trypsin densities, sprayers, and incubation times), and five matrix deposition methods (different sprayers, settings, and matrix concentrations). Evaluation criteria were the number of detected and excluded peaks, percentage of high mass peaks, signal-to-noise ratio, spatial localization, and average intensities of identified peptides, all of which were integrated into a weighted quality evaluation scoring system. Based on these scores, the optimized protocol included an ice-cold EtOH+H2O wash, a 5 min heating step at 95°C, tryptic digestion incubated for 17h at 37°C and CHCA matrix deposited at a final amount of 1.8 μg/mm2. Including a heat-induced protein denaturation step after tissue wash is a new methodological approach that could be useful also for other tissue types. This optimized protocol for spatial peptide detection using MALDI MSI facilitates future biomarker discovery in prostate cancer and may be useful in studies of other tissue types. 相似文献